Construction of VEGF-C siRNA expression vector and its inhibitory effects on the expression of VEGF-C in EC9706 cells

Objective To examine the inhibition of vascular endothelial growth factor C (VEGF-C) by small interfer- ing RNA in human esophageal cancer EC9706 cells.Methods According to the encoding sequences of mRNA of VEGF- C,three pairs of oligonucleotide sequence were designed,synthesized and cloned into siRNA expression vector.The recom- binant VEGF-C-targeted siRNA eukaryotic expression plasmids were transfected into esophageal carcinoma EC9706 cells via lipofectin mediation.Expressions of VEGF-C protein and mRNA in EC9706 cells were assayed by SP immunohistochemis- try,semi-quantitative RT-PCR and in situ hybridization before or after the stable transfection,respectively.Results There were lots of positive granules and positive bands in normal EC9706 cells and in the transfection group cells with trans- fected not-matching any known human coding mRNA.The transfection group cells with transfected by VEGF-C-targeted siRNA expressed few positive granules and weak positive bands.There was significant difference between them (P 0.01).Conclusions siRNA of VEGF-C was constructed.The specific siRNA of VEGF-C can significantly inhibit the ex- pression of VEGF-C in EC9706 cells.The siRNA against VEGF-C maybe serve as a potential method for cancer treatment in human esophageal cancer.