2014•Zhongguo shiyan fangjixue zazhiRequires access
Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury
Pan Ya-la
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Abstract
Objective: To clarify the effect of Jisuikang on neural functional recovery and explore its probable mechanism to improve the axon regeneration microenvironment on protein and mRNA level of nerve growth factor( NGF). Method: The rat models of spinal cord injury( SCI) were established by modified Allen's method. Twenty six rats were randomly allocated into pseudo surgery group( group A),other 130 rats were randomly divided into model group( group B),prednisolone group( group C),high,middle and low doses Jisuikang group( group D-F) after SCI,26 rats in each group. The rats were intervened 30 min after anesthesia recovery. The spinal cord tissue were collected 3,7,14 d after SCI. The changes of the spinal cord tissues were observed through the HE staining,transmission electron microscope( TEM). Immunohistochemistry and RT-PCR were applied to test the expression of protein and mRNA of NGF. Result: HE staining: with the intervention time extending,the neuron survival in group C and E was more obvious than group B. The TEM ultrastructure scoring:group C and E reduced 7 d after injury( P 0. 05). Immunohistochemistry: the different time points expression of NGF of group B-F was significantly higher than group A,the postoperative expression of group C and E remained a high levels after 7-14 d,compared with group B( P 0. 01). RT-PCR: the expression of NGF of group C-F were higher than group B,especially in group C and E. The expression of NGF of group E was obvious higher than other groups after 14 d,compared with group C( P 0. 01). Conclusion: Both Jisuikang and prednisolone can reduce and delay the pathological damage,prevent the occurrence and development of irreversible deformation phenomenon,and promote the expression of NGF after SCI. Prednisolone has a more obvious effect early after SCI,while Jisuikang has a lasting effect. Maybe it is one of the factors of Jisuikang to promote neural functional recovery and improve the axon regeneration microenvironment.