Different doeses of ovalbumin sensitization in airway responsiveness in a murine model of asthma

Objective:To explore the different does of ovalbumin(OVA) sensitization whether have influences on airway hyper responsiveness of mice model of asthma.Methods:The mice were randomly divided into 3 groups,with 10 mice in each group:control group and asthmatic groups(including asthma A group,the does of OVA sensitization was 10 μg every time;and asthma B group,which was 2 mg).The asthmatic A and B group were sensitized on the 0th,14th day with different does of OVA,then inhaled OVA beginning on the 21st day for 6 days.Meanwhile the breathing and the color of oral and tail mucosa were monitered.The last time OVA stimulated and 24 hours later,Penh were measured to evaluate the airway responsiveness by noninvasive lung functional instrument,bronchoalveolar lavage cytology was performed and lung tissue sections were prepared for histopathologic examination to evaluate the airway inflammation.The IgE,IFN-γ of blood and BALF were detected by ELISA method.Results:The skin of mice lip and tail was caynosis after the second allergic sensitization in asthmatic B group mice.The airway hyper responsiveness in asthmatic group was significantly higher than that in the control mice,and asthmatic B group's Penh was significantly higher than asthmatic A group.The total cell number and the eosinophils in BALF of the asthmatic mice were significantly higher than that in the control mice;The total cell number and the eosinophils in BALF of the asthmatic B group were insignificantly higher than that in asthmatic A group.The contents of IgE in peripheral blood and BALF of the asthmatic mice were significantly higher than that in the control mice,and which in the asthmatic B group were significantly higher than that in asthmatic A group.The contents of IFN-γ in peripheral blood and BALF of the asthmatic mice were significantly lower than that in control ones,were insignificantly higher in the asthmatic B group than those in asthmatic A group.Conclusion:A murine model of asthma is successfully established.The one sensitized with high does of OVA tends to have higher responsiveness than the group with low inducing does of OVA.