Effect of Huaiqihuang particles on serum TNF-α,IL-18 and IL-10 in children with primary nephrotic syndrome

Objective To observe the dynamic changes of serum TNF-α,IL-18 and IL-10 in children with primary nephrotic syndrome(PNS) and the effect of Huaiqihuang particles on these inflammatory factors ,infection and relapse of the patients. Methods Totally 67 patients first diagnosed with PNS were randomly divided into group A (35 cases) and group B (32 cases). Group A was treated with prednisone and Huaiqihuang particles,while group B was treated with prednisone only. Observe the serum TNF-α,IL-18 and IL-10 level before the treatment,0.5 month,1 month,3 months and 6 months after treatment. Record the infection and relapse patients within 1 year. Group C was control group (25 healthy children). Serum TNF-α,IL-18 and IL-10 level was detected by ELISA. Results (1)The infection times of group A(0.54 time each patient)were significantly fewer than group B(1.81 time each patient), and the relapse cases of group A(7,37.5%) were fewer than group B(12,37.5%).(2)TNF-α: Before treatment, the serum level was higher than group C(P 0.05); 0.5 month after treatment, it decreased to normal in both group A and B(P 0.05), then maintained at normal level;there were no differences between the two groups. (3)IL-18:Before treatment, the serum level of groups A and B was higher than group C(P 0.05); it decreased significantly after 3 months’treatment (P 0.05)in group A, which was lower than that of group B but still higher than that of group C(P 0.05). (4)IL-10:Before treatment, the serum level of the gwo groups was higher than group C(P 0.05); 0.5 month after treatment, it decreased in both group A and B(P 0.05); there were no differences between the two groups. Six months after treatment, it increased significantly in group A , higher than group B(P 0.05), but still did not reach the normal level. (5)Relapse cases: Serum TNF-αlevel in group A was lower than that of group B(P 0.05). There were no differences between the two groups in IL-18 and IL-10 level. Conclusion TNF-α,IL-18 and IL-10 participate in the pathogenesis of PNS. The imbalance of inflammatory factors and inhibited factors may be one of the pathogenesis of PNS. Huaiqihuang particles can prevent the infection and relapse of PNS children,by reducing the inflammatory effects of IL-18 and enhancing the inhibitory effects of IL-10.