Protective Effects of Ginkgo Biloba Extract 50 Against H_2O_2-induced Apoptosis in Neonatal Rat Cardiomyocytes

Objective To investigate the protective effects of Ginkgo Biloba Extract 50(GBE 50) against H2O2-induced apoptosis in neonatal rat cardiomyocytes.Methods Myocardial cells of neonatal rats were cultured in vitro,0.2 mmol·L-1 H2O2 was used to induce cardiomyocyte apoptosis.Cultured neonatal rat cardiomyocytes were divided into three groups,i.e.control group,H2O2 group,and H2O2 plus 0.2 mg·mL-1 GBE 50 group.The cardiomyocyte viability was analyzed by MTT assay.The expression of Bcl-2,Bax and Caspase-3 were determined by Western blot.Result Compared with control group,H2O2 significantly decreased the viability of cardiomyocytes(P0.01),expression levels of Bcl-2 was down regulated(P0.01),whereas the expression level of Bax and cleaved Caspase-3 were significantly unregulated,and Bcl-2/Bax ratio was decreased(P0.01).Compared with H2O2 group,cardiomyocyte viability was significantly increased in H2O2+GBE 50 group(P0.01),GBE 50 treatment unregulated the expression level of Bcl-2,down regulated expression levels of Bax and cleaved Caspase-3(P0.01),increased Bcl-2/Bax ratio(P0.01).Conclusion Taken together,these results suggest that GBE 50 could protect cultured neonatal rat cardiomyocytes against H2O2-induced apoptosis.