Effect of hypothermia combined with controlled fluid resuscitation on hemorrhagic shock in rats

Objective To explore the effects of hypothermia combined with controlled fluid resuscitation for uncontrolled hemorrhagic shock in different ages of rats. Methods Sixty rats were divided into control group, conventional resuscitation group, mild hypothermia resuscitation group, controlled fluid resuscitation group and hypothermia+controlled fluid resuscitation group by different treatment in the second phase of uncontrolled hemorrhagic-shock model, with 12 rats in each group. We recorded the survival time and 24 h survival rate of rats in each group and compared the blood gas analysis, blood lactate and hematocrit(Hct) level in each group at 90 min of model. Results Hypothermia+controlled fluid resuscitation group had a significantly longer survival time than the controlled fluid resuscitation group,(15.47±5.32) h vs(9.27±4.71) h, P0.01. The 24 h survival rate was 25% in the controlled fluid resuscitation group and 41.7% in the Hypothermia+controlled fluid resuscitation group. Hypothermia+controlled fluid resuscitation group had a minimum amount of fluid [(44.46±6.75) ml/kg] and had a highest PO2 and lowest blood lactate levels at 90 min of model. Conclusion Mild hypothermia combined with controlled fluid resuscitation can effectively improve the prognosis in rats with hemorrhagic shock.