Experimental Study of Protective Effect of Magnesium Sulfate on Secondary Spinal Cord Injury in Rats

objective to discuss the possible mechanism of protective effects of magnesium sulfate on the injuried spinal cord in rats model.Thirty WisterRats were randomly divided into 3 groups:group A(Normal group);group B (Spinal cord injury) that is SCI;group C(Treatment group):SCI with magnesium sulfate treatment.The SCI Animals model was made by using an improved Allen's weight-drop device.After thirty minutes the animals of group B were injected with 1600mg/kg distilled water through Abdominal cavity injection and group C were injected with 1600mg/kg magnesium sulfate.The contents of H_2O、Mg~(2+)、Ca~(2+) in the injuried spinal cord tissues were examined. The histopathology and uhrastructure of injuried spinal cord tissues were observed through light and electron microscopes. The Apoptosis cell were assayed through TUNEL reagent.The contents of H_2O and Ca~(2+) in injuried spinal cord tissues in each SCI group were obviously increased compared to those in normal group(P0.01),and those in group C was less than those in group B(P0.05).the contents of Mg~(2+) obviously decreased(P0.01) in the injuried spinal cord tissues.The damage of structure in group C was less serious than in group B.The number of apoptosis cell in group B was more than that in group C(P0.01).Conclussion:Earlly intervention with magnesium sulfate can decreased the secondary spinal cord injury,Thus the injuried spinal cord were protected effectively.