Antifungal Action in vitro of Extracts from Garlic by Supercritical Fluid Extraction

Objective:To study antifungal action in vitro of extracts from Garlic by supercritical fluid extraction(SFE).Method:The minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of SFE extract of Garlic against 15 strains dermatophytes were determined by media dilution method.Result:MIC of SFE of garlic to Shanghai strain(Trichophyton tousurans) and Wuhan strains(Trichophyton memtigrophytes,Wool microsporum canis,Achromatism trichophyton rubrum) were 3.13 mg·L-1,MIC to Shanghai strains(Plaster microsporum canis,Wool microsporum canis)and Wuhan strain(Wool microsporum canis) were 1.56 mg·L-1,MIC of SFE to Shanghai strains(Trichophyton cerebriforme,Trichophyton rubrum,Trichophyton gypserum) and Wuhan strains(Trichophyton gypserum,Trichophyton rubrum,Microsporum canis,Candida albicans) were less than 0.78 mg·L-1.MBC values were different.Apart from MBC of SFE of garlic to Shanghai strains(Plaster microsporum canis,Epidermophyton floccosum) and Wuhan strains(Trichophyton mentagroph,Epidermophyton floccosum) were 6.25 mg·L-1,MBC to Shanghai strains(Trichophyton tousurans,Wool microsporum canis) andWuhan strains(Trichophyton rubrum,Achromatism trichophyton rubrum,Microsporum canis) were 3.13 mg·L-1,MBC to Trichophyton cerebriforme(Shanghai strain) was 1.56 mg·L-1,MBC to Shanghai strains(Trichophyton rubrum,Trichophyton gypserum) and Wuhan strains(Trichophyton gypserum,Candida albicans) were less than 0.78 mg·L-1.Conclusion:The SFE extract of Garlic showed strong antifungal effect in vitro.