Hormones regulation on cotton somatic embryogenesis

Effects of exogenous hormones on the prolixferentiation of cotton embryogenic callus, the differentiation and development of cotton somatic embryos were studied. The results indicated that most of hormones inhibited the growth and the proliferentiation of cotton embryogenic callus except 2,4-D and BA, and it was influenced by culture time and genotypes. The formation and development of cotton somatic embryos were greatly infuenced by exogenous hormones, 2,4-D inhibited either formation or development of cotton somatic embryos, the embryos which obtained on the medium only with 2,4-D arrested in globular or heart stages; the role of TDZ was similar with 2,4-D, all of somatic embryos which obtained on the medium only with TDZ were arrested in globular stage; GA inhibited the formation of somatic embryos, and was disadvantage to the maturation and germination of cotton somatic embryos; BU-30 did not influence on the differentiation and development of cotton somatic embryos. BIA?ABA?IAA?BA?KT?ZT?2iP promoted the differentiation of cotton somatic embryos, and the role was increase progressively by the above-mentioned sequence, but they were no greatly influenced on the development of embryo. 2iP was the best hormones for the formation and the development of cotton somatic embryo, the increase in the number of embryos per lg callus was 2.852-fold.