Effect of PGEI,on Production of NO and the Activity of eNOS in Human Umbilical Vein Endothelial Cells.

Objective To evaluate the effects of PGE_1 on Production of No and the activity of eNOS in human umbilical vein endothelial cells(HUVECs).Methods Nitric oxide(NO) production and the activity of nitric oxide synthase(NOS) in HUVECs were measured by No and NOS assay kits,after HUVECs incubated with PGE_1 alone at different concentrations(0.04-0.4 μg/ml) and different times(2-4h) or HUVECs incubated with PGE_1 as pretreatment before TNF-α 10ng/ml.Results(1) Activity of eNOS and the expression of NO were up-regulated by PGE_1 in concentration-dependent manner.(2) Short time intervention with PGE_1 had little effects on both activity of eNOS and the expression of NO.However,the expression of NO was increased after 12h incubation and the activity of eNOS was enhanced after 24h treatment.(3) PGE_1 could prevent eNOS activity inhibition induced by TNF-α.Conclusions PGE_1 has a protection effect on endothelial cells by enhancing eNOS activity and increasing NO production,besides,it could prevent eNOS activity inhibition induced by TNF-α.