To exclude the variation of mutations of clusterin-like protein 1gene in Chinese high myopia patients

Objective To screening the variations of clusterin like protein 1 (CLUL1) gene in encoding sequence in Chinese high myopia patients.Methods Genomic DNA was collected from 204 probands with high myopia (≥-6.0 D). The coding sequences of CLUL1 gene in 204 subjects were analyzed by exon by exon PCR heteroduplex SSCP analysis and sequencing.Results It was found only one heterozygous synonymous mutation of CLUL1 gene codon10(GT G → GT T , Val10Val) in one patient with high myopia.It was not found other variations of CLUL1 gene in encoding sequences in Chinese high myopia patients.Conclusion We found no evidence that mutations in the CLUL1 gene on chromosome 18p11.3 which expressed high in retina were responsible for the high myopia in Chinese; The variation of CLUL1 gene in Chinese population is very rare.