Protective Effect of Saponins of Panax notoginseng on Drug-induced Liver Injury in Mice

Objective:To investigate the protective effect and mechanism of saponins of Panax notoginseng(PNS) on drug-induced liver injury in mice.Method:Mice were randomly divided into six groups,control group,model group,low,middle,high-dose(100,200,400 mg·kg- 1) of PNS and bifendate group(200 mg·kg- 1).The model of drug-induced liver injury was induced by intragastric administration of isoniazid(INH) and rifampin(RFP) in mice.The levels of alanine aminotransferase(ALT),aspartate transaminase(AST) in serum and the content of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px) in liver homogenate were assayed by spectrophotometry.The pathological changes of hepatic tissues were observed by hematoxylin and eosin(HE) staining.Result:The liver index(70.84±5.93),(66.38 ±4.33) mg·g- 1of in PNS middle,high-dose groups was lower than that(83.18±6.12) mg·g- 1in the model group(P0.05 or P0.01).Compared with the model group,the levels of ALT(89.71±12.13),(79.58 ±12.54),(65.86 ±13.82) U·L- 1and AST(101.54±14.61),(83.70±9.85),(69.47±15.41)U·L- 1in PNS low,middle,high-dose groups were significantly decreased(P0.05 or P0.01).The content of MDA(7.80±1.21),(7.07±1.17) nmol·mg- 1in PNS middle,high-dose groups was also lower than that(9.62 ±1.68) nmol·mg- 1in the model group(P0.05 or P0.01),while the activity of SOD(119.69 ±14.32),(129.72±20.22) U·mg- 1and GSH-Px(108.02±17.07),(112.72±17.54) U·mg- 1in PNS middle,high-dose groups was higher than that in the model group(P0.05 or P0.01).Hepatic tissues stained with HE showed that PNS could remarkably relieve liver injury.Conclusion:PNS has protective effect on drug-induced liver injury in mice,which may be related to antioxidant effect.