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2011•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination of Sudan Red in Chilli and Related Products by HPLC-DAD

Zhen Yan

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Abstract

The method for determination of Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ in chilli and related products by reversed-phase high performance liquid chromatography was developed.The separation was performed on symmetry C18 column(3.9mm×150mm,5μm)by equal degree elution using acetonitrile’s acidic water as the mobile phase.The flow rate was 1.0mL/min with 230nm as detection wavelength.The results showed that Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ have good linearity in the range from 0 to 5 mg/L.The relative standard deviations of spiked sample were 2.58%,2.25%,2.65% and 2.39%.The detection limits of four Sudan dyes were 20,40,40μg/kg and 60μg/kg.The average recovery of four Sudan Red dyes was 98.67%—100.67%.This method is rapid,sensitive,simple and accurate.

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What this paper is about

The method for determination of Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ in chilli and related products by reversed-phase high performance liquid chromatography was developed.The separation was performed on symmetry C18 column(3.9mm×150mm,5μm)by equal degree elution using acetonitrile’s acidic water as the mobile phase.The flow rate was 1.0mL/min with 230nm as detection wavelength.The results showed that Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ have good linearity in the range from 0 to 5 mg/L.The relative standard deviations of spiked sample were 2.58%,2.25%,2.65% and 2.39%.The detection limits of four Sudan dyes were 20,40,40μg/kg and 60μg/kg.The average recovery of four Sudan Red dyes was 98.67%—100.67%.This method is rapid,sensitive,simple and accurate.

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Available abstract

The method for determination of Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ in chilli and related products by reversed-phase high performance liquid chromatography was developed.The separation was performed on symmetry C18 column(3.9mm×150mm,5μm)by equal degree elution using acetonitrile’s acidic water as the mobile phase.The flow rate was 1.0mL/min with 230nm as detection wavelength.The results showed that Sudan Red Ⅰ,Ⅱ,Ⅲ,Ⅳ have good linearity in the range from 0 to 5 mg/L.The relative standard deviations of spiked sample were 2.58%,2.25%,2.65% and 2.39%.The detection limits of four Sudan dyes were 20,40,40μg/kg and 60μg/kg.The average recovery of four Sudan Red dyes was 98.67%—100.67%.This method is rapid,sensitive,simple and accurate.

Key concepts: Chromatography, Chemistry, Relative standard deviation, High-performance liquid chromatography, Elution, Acetonitrile, Gradient elution, Detection limit

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