Rapid Detection of Cronobacter sakazakii Using a Loop-Mediated Isothermal Amplification (LAMP) Assay

This study aimed to establish a loop-mediated isothermal amplification(LAMP) method for rapid detection of Cronobacter sakazakii.The gene sequences of ompA were aligned using the biologic software and four specific primers used for LAMP were designed and the reaction condition was optimized.DNA templates from different cell concentrations were extracted by boiling,FTA card and kit methods and detected by the LAMP method to obtain detection limits of 104,103 CFU/mL and 103CFU/mL,respectively.Seven of 12 Cronobacter sakazakii isolates investigated were positive while all the others were negative.This method may provide a more simple and rapid approach to detecting Cronobacter sakazakii in comparison with PCR method.