Alleviation effects of spraying chitosan on spinach seedling physiological indexes under chlorpyrifos stress.

In recent years,more attentions have been paid to the chemical pesticide application and its influences on crops.It is well documented that the use of organophosphorus pesticides in vegetables could significantly change some physiological metabolisms,and then growth of vegetables.In the present study we investigated effects of spraying chitosan with different concentration on the activities of SOD,POD,CAT,and APX,O2-·producing rate,and the content of H2O2,MDA,proline,and soluble sugar in spinach seedlings under chloryrifos stress in the open-field.Results showed that spraying with lower chitosan concentrations(50,100 mg·L-1)alleviated damages caused by chloryrifos stress more effectively than that of with other concentration.After spraying with lower concentrations,the SOD activity was increased,O2-·producing rate,MDA,proline,and H2O2 content in spinach seedlings were reduced.The activities of SOD,and POD,the content of H2O2,MDA,proline,and soluble sugar in chitosan spraying treatments could be recovered more earlier from the chlorpyrifos stress to the control level.The effects of spraying chitosan on the activity of SOD,O2-·producing rate,the content of H2O2,proline,and MDA in spinach were more effectively than on the activities of POD,CAT,and APX,and the soluble sugar content.