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2002•Journal of Clinical CardiologyRequires access

Effect of matrine on norepinephrine-induced hypertrophy and expression of myosin heavy chain genes in cultured rat cardiomyocytes

Ruan Chang-wu

Open publisher page 2 citations

Abstract

s Objective:To assess the effect of matrine(MA) on norepinephrine(NE) stimulating hypertrophy and expression of myosin heavy chain(MHC) genes in cultured rat cardiomyocytes. Method: Rat cardiomyocytes were divided into NE group and NE-MA group. By measuring the diameter and number of cardiomyocytes, incorporation of leucine( 3H-Leu) and dot-blot hybridization, observe the effects of matrine on NE stimulating hypertrophy and MHC expression. Result:NE significantly increased the diameter and 3H-Leu incorporation of cardiomyocytes (P 0.05), and induced the expression of β-MHC gene with the decrease of a-MHC, MA can block the effect of NE on MHC gene expression, but no effect of the darmeter and number of cardiomyocytes. Conclution: MA can reverse the pathologic switching of MHC isoform mediated by NE, it may be valuable to prevent myocardial hypertrophy.

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What this paper is about

s Objective:To assess the effect of matrine(MA) on norepinephrine(NE) stimulating hypertrophy and expression of myosin heavy chain(MHC) genes in cultured rat cardiomyocytes. Method: Rat cardiomyocytes were divided into NE group and NE-MA group. By measuring the diameter and number of cardiomyocytes, incorporation of leucine( 3H-Leu) and dot-blot hybridization, observe the effects of matrine on NE stimulating hypertrophy and MHC expression. Result:NE significantly increased the diameter and 3H-Leu incorporation of cardiomyocytes (P 0.05), and induced the expression of β-MHC gene with the decrease of a-MHC, MA can block the effect of NE on MHC gene expression, but no effect of the darmeter and number of cardiomyocytes. Conclution: MA can reverse the pathologic switching of MHC isoform mediated by NE, it may be valuable to prevent myocardial hypertrophy.

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Available abstract

s Objective:To assess the effect of matrine(MA) on norepinephrine(NE) stimulating hypertrophy and expression of myosin heavy chain(MHC) genes in cultured rat cardiomyocytes. Method: Rat cardiomyocytes were divided into NE group and NE-MA group. By measuring the diameter and number of cardiomyocytes, incorporation of leucine( 3H-Leu) and dot-blot hybridization, observe the effects of matrine on NE stimulating hypertrophy and MHC expression. Result:NE significantly increased the diameter and 3H-Leu incorporation of cardiomyocytes (P 0.05), and induced the expression of β-MHC gene with the decrease of a-MHC, MA can block the effect of NE on MHC gene expression, but no effect of the darmeter and number of cardiomyocytes. Conclution: MA can reverse the pathologic switching of MHC isoform mediated by NE, it may be valuable to prevent myocardial hypertrophy.

Key concepts: Matrine, Muscle hypertrophy, Myosin, Myocyte, Gene isoform, Internal medicine, Western blot, Gene expression

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Effect of matrine on norepinephrine-induced hypertrophy and expression of myosin heavy chain genes in cultured rat cardiomyocytes — Research Paper | ScholarLens