Performances of Four Automated Urine Sediment Analyzers in Detecting Urine Red Blood Cells and White Blood Cells

Objective To analyze the performances of four automated urine sediment analyzers(FUS-200,IQ-200,AVE-764B,and UF-500i) in detecting urine red blood cells(RBC) and white blood cells(WBC).Methods The within-run precision,linearity,carryover rate,and anti-interference performance of four automated urine sediment analyzers(FUS-200,IQ-200,AVE-764B,and UF-500i) in detecting urine RBC and WBC were tested.WBC and RBC were detected using these four analyzers in 100 patients with positive urine samples,and the results were compared with those of manual microscopy.Results The within-run CVs for WBC counting and RBC counting respectively were 7.0% and 9.8% for FUS-200,5.8% and 8.2% for IQ-200,6.3% and 4.9% for AVE-764B,and 3.2% and 5.9% for the UF-500i.The carryover rates for RBCs and WBCs were 0.04% and 0.41%,respectively,for IQ-200,0.09% and 0.12% for UF-500i,0 and 0 for AVE-764B,and 0 and 0 for FUS-200.All these four automated urine sediment analyzers showed a good linearity(R20.99) for RBC with 0~15 000/μl range and WBC with 0~10 000/μl range.The results of RBC and WBC counting showed no significant differences between these four analyzersand and manual microscopy(P0.05).However,the detection of RBC/WBC by these four analyzers were interfered by fungi and crystals.Conclusion All these four automated urine sediment analyzers have high precision,good linearity,and low carryover rate in detecting RBC and WBC in urine sediment.They can be used for the screening of clinical specimens,although they still can not replace the microscope examination.