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2007•Lishizhen Medicine and Materia Medica ResearchRequires access

Preparation and Determination of Polysaccharide from Green Tea

Weizheng An

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Abstract

Objective To develop a stable and simple method to measure the polysaccharide in Green Tea.Methods After the conversion coefficient of polysaccharide from Green Tea to glucose was obtained,the content of polysaccharide was measured by phenol-sulfuric acid method.Results The correlation coefficient of standard curve was 0.999 7,the recovery rate was 99.62 % and RSD was 1.94 %.Conclusion The method is convenient,stable and accurate,which can be used for content determination of polysaccharide from Green Tea as well as it' s related products.

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What this paper is about

Objective To develop a stable and simple method to measure the polysaccharide in Green Tea.Methods After the conversion coefficient of polysaccharide from Green Tea to glucose was obtained,the content of polysaccharide was measured by phenol-sulfuric acid method.Results The correlation coefficient of standard curve was 0.999 7,the recovery rate was 99.62 % and RSD was 1.94 %.Conclusion The method is convenient,stable and accurate,which can be used for content determination of polysaccharide from Green Tea as well as it' s related products.

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Available abstract

Objective To develop a stable and simple method to measure the polysaccharide in Green Tea.Methods After the conversion coefficient of polysaccharide from Green Tea to glucose was obtained,the content of polysaccharide was measured by phenol-sulfuric acid method.Results The correlation coefficient of standard curve was 0.999 7,the recovery rate was 99.62 % and RSD was 1.94 %.Conclusion The method is convenient,stable and accurate,which can be used for content determination of polysaccharide from Green Tea as well as it' s related products.

Key concepts: Polysaccharide, Green tea, Chemistry, Chromatography, Correlation coefficient, Recovery rate, Content determination, Sulfuric acid

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