Experimental Study on the Relationship Between Substantia Nigral Apoptosis and Expression of Bax Protein in Rats with Parkinson Disease

Objective:To study the induction of apoptosis of substantia nigra by 6-OHDA evoke and the relationship between substantia nigral apoptosis and expression of Bax protein.Methods:Seventy-two Wistar male rats were randomly divided into control group(36) and Parkinson disease model group(PD group,36).Using(TUNEL) method,in situ hibridization and electron microscopic techniques,the number of nigral apoptosis and the changes of ultrastructure were observed 1,7,14,21 days after stereotaxic injection of 6-OHDA into right side of the mesencephic ventral tegmental area(VTA) and substantia nigra pars compacta(SNc) of rats.The expression of Bax protein was detected.Results:The number of substantia nigral apoptosis in the PD group was much more than that in the control group,and from 1st to 21st day the number of substantia nigral apoptosis was increased gradualy.In the PD group the ultrastructure of substantia nigra exhibited morphological characteristics of apoptosis.The expression of Bax protein attained the peak value promptly at 1st day,and then decreased gradually.Bax protein in the PD group was higher than in the control group.Conclusion:Apoptosis of substantia nigra can be induced by 6-OHDA.Apoptosis of substantia nigral induced by 6-OHDA contributes to the pathogenesis of PD.As a key evoking factor,Bax protein involves in pathogenesis of PD.