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2011•Zhongguo fushe weishengRequires access

Radiosensitivity Evaluation of Human Tumor Cell Lines by Single Cell Gel Electrophoresis

Yan Wang

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Abstract

Objective To explore the feasibility of determining radiosensitivity of human tumor cell lines in vitro using single cell gel electrophoresis(SCGE).Methods Three human tumor cell lines were selected in this study,HepG2、EC-9706 and MCF-7.The surviving fraction(SF)and DNA damage were detected by MTT assay,nested PCR technique and comet assay respectively.Results MTT assay:The SF of HepG2 and EC-9706 after irradiated by 2、4and 8Gy was lower significantly than that of MCF-7,which showed that the radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7.But there was no statistical difference of SF between HepG2 and EC-9706.SCGE: The difference of radiosensitivity among these three tumor cell lines was significant after 8Gy γ-ray irradiation.Conclusion The multi-utilization of many biological parameter is hopeful to evaluate the radiosensitivity of tumor cells more objectively and exactly.

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What this paper is about

Objective To explore the feasibility of determining radiosensitivity of human tumor cell lines in vitro using single cell gel electrophoresis(SCGE).Methods Three human tumor cell lines were selected in this study,HepG2、EC-9706 and MCF-7.The surviving fraction(SF)and DNA damage were detected by MTT assay,nested PCR technique and comet assay respectively.Results MTT assay:The SF of HepG2 and EC-9706 after irradiated by 2、4and 8Gy was lower significantly than that of MCF-7,which showed that the radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7.But there was no statistical difference of SF between HepG2 and EC-9706.SCGE: The difference of radiosensitivity among these three tumor cell lines was significant after 8Gy γ-ray irradiation.Conclusion The multi-utilization of many biological parameter is hopeful to evaluate the radiosensitivity of tumor cells more objectively and exactly.

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Available abstract

Objective To explore the feasibility of determining radiosensitivity of human tumor cell lines in vitro using single cell gel electrophoresis(SCGE).Methods Three human tumor cell lines were selected in this study,HepG2、EC-9706 and MCF-7.The surviving fraction(SF)and DNA damage were detected by MTT assay,nested PCR technique and comet assay respectively.Results MTT assay:The SF of HepG2 and EC-9706 after irradiated by 2、4and 8Gy was lower significantly than that of MCF-7,which showed that the radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7.But there was no statistical difference of SF between HepG2 and EC-9706.SCGE: The difference of radiosensitivity among these three tumor cell lines was significant after 8Gy γ-ray irradiation.Conclusion The multi-utilization of many biological parameter is hopeful to evaluate the radiosensitivity of tumor cells more objectively and exactly.

Key concepts: Radiosensitivity, Comet assay, Molecular biology, Cell culture, Gel electrophoresis, MTT assay, Biology, Cell

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