Experimental StudyIon the Synergia Theory of Processing with Vinegar——Effects of Repetitive Processing with High Dose Vinegar on the Components of Rhizoma Corydalis

Objective:To study the effects of quantity of processing vinegar and processing methods on the components of Rhizoma Corydalis.Methods: HPLC was used to detect the content of tetrahydropalmatine and protopine in processing products of Rhizoma Corydalis.The column was Hypersil BDS(250 mm×4.6 mm,5 μm),the flow rate was 1.0 mL·min~(-1).For tetrahydropalmatine,mobile phase was methanol-0.1% phosphoric acid(adjust PH 6.0 with triethylamine)(65∶35),The UV detector was set at 280 nm;For protopine,mobile phase was acetonitrile-triethylamine acetic acid,The UV detector was set at 289 nm.Results: The extraction solubility of tetrahydropalmatine and protopine in Rhizoma Corydalis processed with 30% vinegar(100 g Rhizoma Corydalis with 30 mL vinegar) is the hightest,vinegar-sauted has better effects than vinigar-boiled.Conclusion: The components of Rhizoma Corydalis by repetitive processed with high dose vinegar or boiled in vinegar for long time was decreased.