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Interaction between curcumin and bovine serum albumin by fluorescence

Lan Rui-jia

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Abstract

Objective To study the mechanism of interation between curcumin(CU) and bovine serum albumin(BSA).Methods The style of quenching was investigated by changing the reaction temperature.The number and constant of binding sites of CU to BSA were calculated.Results Static quenching was the main reason that caused the quenching of BSA fluorescence.The apparent combining constants(K0) were 5.26 × 106 at 21 ℃ and 4.60 × 106 at 31 ℃,and the number of binding sites(n) was 1.35.By calculating the thermodynamic parameters,it can be deduced that hydrophobic force is the main binding force between CU and BSA.Conclusion Fluorometric analysis is a good method for the study on CU-BSA binding reaction.It has the advantages of high speed and high sensitivity.

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What this paper is about

Objective To study the mechanism of interation between curcumin(CU) and bovine serum albumin(BSA).Methods The style of quenching was investigated by changing the reaction temperature.The number and constant of binding sites of CU to BSA were calculated.Results Static quenching was the main reason that caused the quenching of BSA fluorescence.The apparent combining constants(K0) were 5.26 × 106 at 21 ℃ and 4.60 × 106 at 31 ℃,and the number of binding sites(n) was 1.35.By calculating the thermodynamic parameters,it can be deduced that hydrophobic force is the main binding force between CU and BSA.Conclusion Fluorometric analysis is a good method for the study on CU-BSA binding reaction.It has the advantages of high speed and high sensitivity.

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Available abstract

Objective To study the mechanism of interation between curcumin(CU) and bovine serum albumin(BSA).Methods The style of quenching was investigated by changing the reaction temperature.The number and constant of binding sites of CU to BSA were calculated.Results Static quenching was the main reason that caused the quenching of BSA fluorescence.The apparent combining constants(K0) were 5.26 × 106 at 21 ℃ and 4.60 × 106 at 31 ℃,and the number of binding sites(n) was 1.35.By calculating the thermodynamic parameters,it can be deduced that hydrophobic force is the main binding force between CU and BSA.Conclusion Fluorometric analysis is a good method for the study on CU-BSA binding reaction.It has the advantages of high speed and high sensitivity.

Key concepts: Bovine serum albumin, Quenching (fluorescence), Chemistry, Binding constant, Curcumin, Fluorescence, Serum albumin, Chromatography

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