Zhizhong Cui
Abstract
With nonradioactive digoxigenin-labeled serotype I-specific MDV DNA fragment as probe, the hybridization could effectively detect serotype Ⅰ MDV DNA fixed on nitrocellulose filters by Southern blot or dot blot. Its specificity and sensitivity was as good as that with ~(32)P-labeled probe.
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With nonradioactive digoxigenin-labeled serotype I-specific MDV DNA fragment as probe, the hybridization could effectively detect serotype Ⅰ MDV DNA fixed on nitrocellulose filters by Southern blot or dot blot. Its specificity and sensitivity was as good as that with ~(32)P-labeled probe.
Key concepts: Digoxigenin, Dot blot, Hybridization probe, DNA, Molecular biology, Southern blot, Nitrocellulose, Virology