Effects of JWSNS on plasma adrenocorticotropic hormone and cortisol,and gastric tissue of chronic psychological stress rat models

Objective To study the effects of JWSNS exert on behavior and gastric mucosa of stress rat model of chronic stress ulcer,and to elucidate the mechanism of stress-induced gastrointestinal dysfunction.Methods Sixty Wistar rats were randomly divided into 6 groups: normal group,model group,omeprazole group,and large,median,small dose JWSNS groups.The subjects were stimulated with 10 kinds of unpredictable stimuli for 4 weeks for construction of chronic psychological stress gastric ulcer model except for those in normal group.Since the 3rd week 0.25,0.5,and 1 g/mL JWSNS and 0.3 mg/mL omeprazole were given to the subjects in large,median,small dose JWSNS groups,and omeprazole group,respectively,by 2 mL daily;whereas 2 mL saline was given instead in normal group and model group.Behavior observation and gastric mucosal ulcer index,plasma ACTH and CORT,and conventionally HE staining were carried out for analysis.Results Compared with model group,vertical and cross grid movement counts of rats from normal group and JWSNS groups were significantly increased(P0.05,P0.01).Compared with model group,significant alleviation of gastric pH value and mucosal ulcer index were revealed in JWSNS groups and omeprazole group,with significant reduction in ACTH and CORT(P0.05,P0.01).So was between small JWSNS group and high or median JWSNS group(P0.05,P0.01).Diffuse bleeding ulcer was observed in model group,whereas it was alleviated in JWSNS groups.Conclusion JWSNS can enhance anti-stress adaptive capacity in stressed rat model,improve mood,reduce stress-induced gastric mucosal injury,inhibit gastric acid secretion,and regulate the state of HPA axis.