Effect of Shenqingyin on Expression of Collagen I and TGF-β_1 in Rats with Renal Interstitial Fibrosis

Objective:To study the effect and mechanism of Shenqingyin(SQY,a herbal compound) on renal interstitial fibrosis rats.Methods:Fourty male SD rats were randomly divided into 5 groups:normal group,model group,SQY prevention group,SQY treatment group and Benazepril treatment group.he models of renal interstitial fibrosis were induced by intragastric administration of adenine.At the end of 6th week,the blood samples were taken to check endothelin-1(ET-1) level;Then rats were sacrificed,whose renal tissues were taken for pathological examination by HE staining and Masson staining;Immunohistochemistry was used to measure the expressions of collagenⅠ(ColⅠ) and TGF-β1 in nephridial tissue.Results:The degree of renal interstitial fibrosis in SQY intervened groups were more slightly than that in the model group and the control group.The level of ET-1 or the expressions of collagenⅠand TGF-β1 in SQY intervened groups were lower than those in the model group and the control group(P0.05).Conclusion:SQY has an obvious relieving effect on renal interstitial fibrosis.The mechanism was probably that SQY decreased the expressions of collagenⅠ and TGF-β1 in the nephridial tissues and lowered the ET-1 level in blood plasma.