Separation and Purification of Small-Molecule Pe ptides with Immunomodulating Activity from Goat Placenta

The purpose of this study was to isolate and purify small-molecule peptides with immunomodulating activity from goat placenta.Ultrafiltration fraction from goat placenta was prepared by a conventional metho d,followed by isolation,purification and analysis by reversed phase-high performance liquid chromatography(RT-HPLC).The immunomodulating activity of its components was measured by E-rosette test and the N-terminal sequence was measured by Edman degradation react ion.Three small-molecule peptides with high purity were obtained,two of which were immune-active.The results of sequencing and retrieving showed that they were new peptides.RT-HPLC was one of the optimal m ethods to purify and analyze bioactive peptides.The small-molecule peptides from goat placenta revealed strong immune activity.