Effects of insulin-like growth factor 1 on the myocardium regeneration and anti-inflammation in mesenchymal stem cells

Objective:To investigate the effect of pretreatment with insulin-like growth factor-1(IGF-1) on the myocardium regeneration and anti-inflammation in mesenchymal stem cells(MSCs).Method:MSCs were treated with 10 μg/L IGF-1 for 48 hours.Chemokine receptor CXCR4 was analyzed with flow cytometry.Rats' myocardial infarction(MI) model was made,100 μl DMEM basal medium without cells(Control group) or containing 1×107 MSCs treated with or without IGF-1(IGF-1-MSCs group and MSCs group) were infused into the MI rats via the tail vein.After 4 weeks of MSCs transplantation,inflammation factors(TNF-α,IL-1β and IL-6),homing of engrafted MSCs,CX43,cardiac-specific protein Troponin T protein were measured.Result:After pretreatment on MSCs with IGF-1,flow cytometric analysis showed marked enhancement of expression of CXCR4 in the cell surface.After 4 weeks of transplantation,we found:①a greater number of engrafted MSCs arrived and survived in the peri-infarct region(P0.05),②TnT positive(P0.05) and CX43(P0.05) in infracted around area were enhanced,③expression of inflammation factors(TNF-α,IL-1β and IL-6) were decreased significantly.Conclusion:IGF-1 improves myocardium regeneration and anti-inflammation of MSCs transplanted in a rat model of myocardial infarction.