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cDNA Cloning and Sequencing of Human Survivin Gene

Jingyu Zhang

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Abstract

Objective:To study the cDNA cloning and sequencing of human survivin gene.Methods:Total RNA was extracted from fetal spleen tissue of Chinese race and the cDNA of survivin gene was amplified by reverse transcription_polymerase chain reaction(RT_PCR)from RNA template.The cDNA fragment was cloned into pUC18plasmid and sequenced by dideoxy_nucleotide chain termination method.Results:The cDNA seˉquence showed that only one base was different from that in Gene Bank,and the difference led to the change of amino acid.Conclusion:The cDNA of survivin gene was cloned successfully and one base pair different from that of other nations is found.

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What this paper is about

Objective:To study the cDNA cloning and sequencing of human survivin gene.Methods:Total RNA was extracted from fetal spleen tissue of Chinese race and the cDNA of survivin gene was amplified by reverse transcription_polymerase chain reaction(RT_PCR)from RNA template.The cDNA fragment was cloned into pUC18plasmid and sequenced by dideoxy_nucleotide chain termination method.Results:The cDNA seˉquence showed that only one base was different from that in Gene Bank,and the difference led to the change of amino acid.Conclusion:The cDNA of survivin gene was cloned successfully and one base pair different from that of other nations is found.

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Available abstract

Objective:To study the cDNA cloning and sequencing of human survivin gene.Methods:Total RNA was extracted from fetal spleen tissue of Chinese race and the cDNA of survivin gene was amplified by reverse transcription_polymerase chain reaction(RT_PCR)from RNA template.The cDNA fragment was cloned into pUC18plasmid and sequenced by dideoxy_nucleotide chain termination method.Results:The cDNA seˉquence showed that only one base was different from that in Gene Bank,and the difference led to the change of amino acid.Conclusion:The cDNA of survivin gene was cloned successfully and one base pair different from that of other nations is found.

Key concepts: Complementary DNA, Rapid amplification of cDNA ends, Cloning (programming), Molecular biology, Gene, Survivin, Biology, cDNA library

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