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2011•Journal of Biological ControlRequires access

Selection of Antagonistic Bacteria against Citrus Green Mold and Its Inhibition Activity on Shatang Mandarin Fruit

Liu Yang

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Abstract

A bacterial strain HC-03,isolated from rhizosphere soil of citrus farms,exhibited extensive antagonistic effect on 12 fruit pathogens.The strain was identified as Bacillus amyloliquefaciens based on 16S rDNA sequence homology,specific PCR detection,physiological and biochemical characteristics and morphological observation.Effect of different treatments of strain HC-03 against disease caused by Penicillium digitatum on inoculated shatang mandarin was also investigated.Results showed that citrus green mold incidence of shatang mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HC-03 were all less than 20%,and lesion diameter were all below 10 mm.

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What this paper is about

A bacterial strain HC-03,isolated from rhizosphere soil of citrus farms,exhibited extensive antagonistic effect on 12 fruit pathogens.The strain was identified as Bacillus amyloliquefaciens based on 16S rDNA sequence homology,specific PCR detection,physiological and biochemical characteristics and morphological observation.Effect of different treatments of strain HC-03 against disease caused by Penicillium digitatum on inoculated shatang mandarin was also investigated.Results showed that citrus green mold incidence of shatang mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HC-03 were all less than 20%,and lesion diameter were all below 10 mm.

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Available abstract

A bacterial strain HC-03,isolated from rhizosphere soil of citrus farms,exhibited extensive antagonistic effect on 12 fruit pathogens.The strain was identified as Bacillus amyloliquefaciens based on 16S rDNA sequence homology,specific PCR detection,physiological and biochemical characteristics and morphological observation.Effect of different treatments of strain HC-03 against disease caused by Penicillium digitatum on inoculated shatang mandarin was also investigated.Results showed that citrus green mold incidence of shatang mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HC-03 were all less than 20%,and lesion diameter were all below 10 mm.

Key concepts: Biology, Bacillus amyloliquefaciens, Rhizosphere, Penicillium digitatum, Strain (injury), Inoculation, Mold, 16S ribosomal RNA

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