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2003•Chinese Journal of Oral & Maxillofacial SurgeryRequires access

EFFECTS OF MECHANICAL STRAIN ON ALP ACTIVITY AND COLLAGEN I EXPRESSION IN HUMAN OSTEOBLASTS

Zou Shu

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Abstract

Objective To study the changes in ALP activity and collagen I expression in osteoblasts after mechanical strain stimulation. Methods MG 63 human osteoblasts were cultured, and exposed to mechanical strain using Flexercell Stretching Unit with different stimulation times. ALP activity and collagen I mRNA expression were detected by assay kit and RT PCR reaction. Results ALP activity was increased significantly after mechanical stimulation. The expression of collagen I was decreased in the period of 6 to 12 h, but increased during 24 to 48 h in strained cells. Conclusion This study suggest that mechanical strain may not only increased the ALP activity, but also alter the mRNA expression of collagen I in human osteoblasts.

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Objective To study the changes in ALP activity and collagen I expression in osteoblasts after mechanical strain stimulation. Methods MG 63 human osteoblasts were cultured, and exposed to mechanical strain using Flexercell Stretching Unit with different stimulation times. ALP activity and collagen I mRNA expression were detected by assay kit and RT PCR reaction. Results ALP activity was increased significantly after mechanical stimulation. The expression of collagen I was decreased in the period of 6 to 12 h, but increased during 24 to 48 h in strained cells. Conclusion This study suggest that mechanical strain may not only increased the ALP activity, but also alter the mRNA expression of collagen I in human osteoblasts.

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Available abstract

Objective To study the changes in ALP activity and collagen I expression in osteoblasts after mechanical strain stimulation. Methods MG 63 human osteoblasts were cultured, and exposed to mechanical strain using Flexercell Stretching Unit with different stimulation times. ALP activity and collagen I mRNA expression were detected by assay kit and RT PCR reaction. Results ALP activity was increased significantly after mechanical stimulation. The expression of collagen I was decreased in the period of 6 to 12 h, but increased during 24 to 48 h in strained cells. Conclusion This study suggest that mechanical strain may not only increased the ALP activity, but also alter the mRNA expression of collagen I in human osteoblasts.

Key concepts: Stimulation, Strain (injury), Chemistry, Messenger RNA, Osteoblast, Endocrinology, Internal medicine, Gene expression

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