Studies on Transfection of Anti-swine Fever Virus Gene into Mouse Embryonic Fibroblasts (MEF) Mediated by LiPosome

The study aimed to investigate the conditions of transfection of the anti-classical swine fever virus gene recombination Plasmid PGPUG/GFP/Neo into the mouse embryonic fibroblasts mediated by liPosome,in order to Provide the technical Platform for construction of animal disease model and transgenic Pigs against classical swine fever virus.In this study,different methods of isolation and culture of mouse embryonic fibroblasts were comPared,and transfected the anti-classical swine fever virus gene recombination Plasmid PGPUG/GFP/Neo into the 3 to 5 generation of fetal fibroblast cells mediated by liPosome(LiPofectamineTM 2000).The results showed that,in different concentrations of liPosomes,recombinant Plasmid and different combinations of transfection time used in this sdudy,it was obtained 19% transfection rate in 2 μL liPosome and 1.5 μg recombinant Plasmid,incubated 6 h of combination,it was significantly higher than the other combinations(P0.05).The results indicated that,the anti-classical swine fever virus gene recombination Plasmid PGPUG/GFP/Neo could be effectively transfected into mouse embryonic fibroblasts mediated by oPtimal combinations of liPosomes,recombinant Plasmid and transfection time.