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Zinc Finger Nucleases and Targeted Genome Engineering in Plants

Yun Song, Qiao Yonggang, Guiquan Li

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Abstract

Zinc finger nucleases(ZFNs) typically consist of three to four zinc fingers(ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokⅠ.In this configuration,the ZFs constitute the binding module and the FokⅠ domain the cleavage module.ZFNs offer a general way to deliver a targeted site-specific double strand break(DSB) to the genome.DSBs are mainly repaired by two pathways,non-homologous end joining(NHEJ) and homologous recombination(HR),which targeted mutagenesis will be generated.In this review,we are first describe the structure of ZFNs,and ZFN-mediated targeted genome engineering via HR and NHEJ,and the methods of construct novel ZFNs and the steps of ZFN-mediated gene targeting.Then we mainly explore that ZFNs were used in plants.

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What this paper is about

Zinc finger nucleases(ZFNs) typically consist of three to four zinc fingers(ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokⅠ.In this configuration,the ZFs constitute the binding module and the FokⅠ domain the cleavage module.ZFNs offer a general way to deliver a targeted site-specific double strand break(DSB) to the genome.DSBs are mainly repaired by two pathways,non-homologous end joining(NHEJ) and homologous recombination(HR),which targeted mutagenesis will be generated.In this review,we are first describe the structure of ZFNs,and ZFN-mediated targeted genome engineering via HR and NHEJ,and the methods of construct novel ZFNs and the steps of ZFN-mediated gene targeting.Then we mainly explore that ZFNs were used in plants.

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Available abstract

Zinc finger nucleases(ZFNs) typically consist of three to four zinc fingers(ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokⅠ.In this configuration,the ZFs constitute the binding module and the FokⅠ domain the cleavage module.ZFNs offer a general way to deliver a targeted site-specific double strand break(DSB) to the genome.DSBs are mainly repaired by two pathways,non-homologous end joining(NHEJ) and homologous recombination(HR),which targeted mutagenesis will be generated.In this review,we are first describe the structure of ZFNs,and ZFN-mediated targeted genome engineering via HR and NHEJ,and the methods of construct novel ZFNs and the steps of ZFN-mediated gene targeting.Then we mainly explore that ZFNs were used in plants.

Key concepts: Zinc finger nuclease, Genome engineering, Zinc finger, Genome editing, Homologous recombination, Non-homologous end joining, DNA, Genetics

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