The Effect of Inhibitory Expression of c-myc Gene by siRNA on Cervical Carcinoma Cell Proliferation

Objective To investigate the effect of small interference RNA targeting c-myc gene on HeLa cells proliferation.Method The sequence of Small interfering RNA(siRNA)targeting c-myc gene was designed and DNA template was synthesized,then siRNA was obtained by in vitro transcription.Blank control cells and cells treated with siRNA-scr were taken as controls.After ds-siRNA was transfected into HeLa cells with cationicpolymer jetSITM-ENDO,the proliferation of HeLa cells was detected by cell counting.The change of cell cycle and protein level was determined by Flow cytometry method.The inhibitory effect of siRNA on mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR).Results①Cell counting results showed that siRNA targeting c-myc signif- icantly inhibited the proliferation of cervical carcinoma cells,HeLa cells,while the control siRNA-scr had no effect on HeLa cells.②Flow cytometry assay showed that c-myc siRNA affected the distribution of HeLa cells cell cycle,the cell population increased in G0/G1 phase and decreased in S phase.③Flow cytometry assay also showed that the siRNA targeting human c-myc gene effectively inhibited the expression of c-myc.④RT-PCR results showed that c-myc mRNA expression level decreased after transfection of c-myc siRNA, while c-myc mRNA expression level of blank control cells,cells treated with jetSITM-ENDO and cells treated with siRNA-scr had no change.Conclusions The siRNA targeting human e-myc gene reduced c-myc expression on protein and mRNA level,and inhibited HeLa cells proliferation.