Identification of the Pathogen Causing Mung Bean Verticillium wilt in Xinjiang

【Objective】Since 2013,a disease of chlorosis,yellowing,wilting of leaves,and dying late was observed on mung bean in plantation of experiment station of agricultural college of Shihezi university and the 142 th farm regiment in Xinjiang,Vascular discoloration was observed when plants were dissected,and it was suspected a Verticillium wilt. In order to find out the pathogenic species,the identification was conducted.【Method】Tissue isolation was used to isolate mung bean Verticillium wilt diseased plant samples and obtain pure culture strains,paper pots tear bottom dipping root methods were used to definitude pathogenicity of the isolations,morphological and molecular methods were used to identify the isolations.【Result】Colony morphology,conidiophores and conidia of the isolates with the reported Verticillium dahliae was consistent,molecular biology identification showed sequence up to 99. 58% homology with that of a China isolate of V. dahliae( EU835817. 1).【Conclusion】Disease of chlorosis,yellowing,wilting of leaves,vascular discoloration and dying late occurred on mung bean in Shihezi area is mung bean Verticillium wilt by V. dahliae. Because Verticillium wilt is a typical soil- borne disease,mung bean should be avoided in the rotation crop field with Verticillium wilt to shun off losses.