Study on Extraction of Anthocyanins in Bilberry with Enzyme and Acid
Li‐Xin Hu
Abstract
Li‐Xin Hu
Abstract
The enzyme and acid was used to extract anthocyanins in bilberry,and then the ratio of solid to liquid,the amount of enzyme,the temperature,the pH and the time of enzymolysis were investigated.Through several groups of different experiments on the extraction process,the optimal parameters were obtained as follow:the ratio of solid to liquid was 1:10(g:g),the amount of enzyme was 2 %,pH was 3.5,zymolytic temperature was 55 ℃ and the time of enzymolysis was 60 minutes.Based on the above conditions,the content of anthocyanins in extracted liquid was 8.92 mg,and the extract had a good anti-oxidative activity.
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The enzyme and acid was used to extract anthocyanins in bilberry,and then the ratio of solid to liquid,the amount of enzyme,the temperature,the pH and the time of enzymolysis were investigated.Through several groups of different experiments on the extraction process,the optimal parameters were obtained as follow:the ratio of solid to liquid was 1:10(g:g),the amount of enzyme was 2 %,pH was 3.5,zymolytic temperature was 55 ℃ and the time of enzymolysis was 60 minutes.Based on the above conditions,the content of anthocyanins in extracted liquid was 8.92 mg,and the extract had a good anti-oxidative activity.
Key concepts: Bilberry, Chemistry, Extraction (chemistry), Enzyme, Chromatography, Enzyme assay, Food science, Biochemistry