1999•Chinese Journal of Current Advances in General SurgeryRequires access
BIOLOGICAL CHARACTERITICS OF THE HUMAN GASTRIC CANCER CELL LINE TRANSFECTED WITH CASPASE-1 GENE
Ji-de Hua
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Abstract
Objective :To investigate the biological characteristics of human gastric cancer transfected with Caspase-1 gene. Methods : Recombinant retroviral vector with human Caspase-1 cDNA was transferred into human gastric cell line X-108.The proliferation tumorigenicity, cycle of the delivered cell line and the CEA concertration in the super-nate of cell culture were analyzed, respectively. Results: Apoptosis was not induced, but the cell number of G1 stage increased, the S stage decreased, the CEA concentration in supernate also decreased, the proliferation and the tumorigenicity cf the gastric cell line transfected with Caspase-1 gene were considerably inhibited. Conclusion : Though apoptosis of the gastric cancer cell line X-108 transfected with Caspase-1 gene was not induced,the proliferation was inhibited.