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2008•Chinese Journal of Analysis LaboratoryRequires access

Study on spectrophotometric determination of proteins by using chrome azurol SCu(II) complex as spectral probe

Xiaohua Wu

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Abstract

A new mothod for the determination of proteins by using chrome azurol S-Cu(Ⅱ) complex as spectral probe has been established.It is based on the binding interaction of protein with chrome azurol S-Cu(Ⅱ) complex in Briton-Robinson buffer at pH 5.0.The binding interaction produces supermolecular complex chrome azurol S-Cu(Ⅱ)-BSA with its maximum absorption at 620 nm.The apparent molar absorptivity of this complex is 6.13×105 L·mol-1·cm-1.Beer′s law is obeyed in the range of 10～60 μg/mL for BSA.The method has been applied to the direct determination of proteins in the egg albumin with the recoveries of 100%～105%.

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What this paper is about

A new mothod for the determination of proteins by using chrome azurol S-Cu(Ⅱ) complex as spectral probe has been established.It is based on the binding interaction of protein with chrome azurol S-Cu(Ⅱ) complex in Briton-Robinson buffer at pH 5.0.The binding interaction produces supermolecular complex chrome azurol S-Cu(Ⅱ)-BSA with its maximum absorption at 620 nm.The apparent molar absorptivity of this complex is 6.13×105 L·mol-1·cm-1.Beer′s law is obeyed in the range of 10～60 μg/mL for BSA.The method has been applied to the direct determination of proteins in the egg albumin with the recoveries of 100%～105%.

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Available abstract

A new mothod for the determination of proteins by using chrome azurol S-Cu(Ⅱ) complex as spectral probe has been established.It is based on the binding interaction of protein with chrome azurol S-Cu(Ⅱ) complex in Briton-Robinson buffer at pH 5.0.The binding interaction produces supermolecular complex chrome azurol S-Cu(Ⅱ)-BSA with its maximum absorption at 620 nm.The apparent molar absorptivity of this complex is 6.13×105 L·mol-1·cm-1.Beer′s law is obeyed in the range of 10～60 μg/mL for BSA.The method has been applied to the direct determination of proteins in the egg albumin with the recoveries of 100%～105%.

Key concepts: Chemistry, Molar absorptivity, Spectrophotometry, Complex formation, Absorption (acoustics), Quantitative analysis (chemistry), Chromatography, Nuclear chemistry

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