Studies on Transgenic Acceptor System of Ground-Cover Chrysanthemum Via Indirect Somatic Embryogenesis

In this paper transgenic acceptor systems of chrysanthemum cv.'Yurenmian' via indirect somatic embryogenesis were established by three steps cultivation.The authors studied the effects of hormones,illumination intensity on indirect somatic embryogenesis through stem segments(internodal segments)as explants.Results showed that:embryogenic calli induction medium was MS+KT 2.0 mg· L-1+2,4-D 2.0 mg·L-1+NAA 0.5 mg·L-1,15 days later,yellow greenish,compact nodular calli were transferred embryogenic calli differentiation medium MS plus KT 2.0 mg·L-1,2,4-D1.0 mg·L-1and NAA 0.5 mg·L-1 for 15 days,and were transferred differentiation medium MS plus KT 2.0 mg·L-1and NAA 0.5 mg·L-1 for 20 days.Illumination intensity was 1 000～2 000 lx.The highest rate of embryogenic calli reached 95.3%,the highest rate of embryogenic calli differentiation reached 92.7%,average number of shoots per stem segment explant was 17.8,and stability of regeneration shoots reached 99.5%.Experiments of the sensitivity of antibiotics for transgenic acceptor systems of chrysanthemum cv.'Yurenmian' via indirect somatic embryogenesis showed:the selection concentrations of kanamycin was 10 mg·L-1,the selection concentrations of cefotaxime was 300 mg·L-1 while embryogenic calli were induced and 100 mg·L-1 while embryogenic calli differentiation.