The alcohol extraction,identification and effect of the antibacterial components of Houttuynia cordata Thunb

The conditions of the alcohol extraction from Houttuynia Cordata Thunb was optimized through orthogonal experiment indexed by the bacteriostatic effect.And the products were purified by liquid-liquid extraction and its components were analyzed by GC-MS and HPLC.At the same time,its antibacterial effect was tested.The result showed that the optimized conditions were 70 % alcohol,80 ℃,4 h,and feed radio 1︰15.Only the liquid purified by chloroform was effective.The GC-MS and HPLC analysis showed that the liquid extracted by chloroform contained 13 phenolic compounds and a little of sodium houttuyfonate and 2-Undecanone.The microbial resistance test proved that the antibacterial components were highly effective against Escherichia coli(MIC:16.0 mg/mL),Staphylococcus aureus(MIC:8.0 mg/mL),Candida albicans(MIC:4.0 mg/mL),Aspergillus flavus(MIC:8.0 mg/mL) and Aspergillus niger(MIC:8.0 mg/mL).