The Relationship between Mating Factors with Mycelial Growth Rate of Agrocybe salicacola (Bolbitiaceae)

Basidiospores of Agrocybe salicacola were collected from a mature fruiting body by ejection. 224 single-spore strains were isolated respectively by dilution separation method,210 were identified as monocaryons. The result of mating test suggested that the sex-inheritance of A.salicacola is tetrapolar heterothallism. Four different mating-type strains were acquired by mating test,and the ratio of AxBx:AxBy:AyBx:AyBy of monocaryotic strains was 47:59:53:51. The results showed that the number and distribution of its mating types was related with spore germination,growth rate of spore germination and mycelium growth. In four mating type strains,the growth rate of one mating type strains was more slow than other three,and only there were 3 and 5 slow-growing strains in other two mating type strains. Especially,although the growth rates between the Fast-Fast mating heterocaryotic strains and Fast-Slow mating heterocaryotic strains in YPD plate were primarily same,but the differences in cultivation bag were obvious. The growth rate genes may be linkaged with mating factors A and B. In breeding of mushroom,most scientists may select Fast-Slow mating heterocaryon,so distorted segregation of mating types may happen.