2011•Food ScienceRequires access
Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides
Gou Xiao-jun
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Abstract
Tartary buckwheat polysaccharides(TBPs) were extracted by boiling water,precipitated by alcohol and purified by ion-exchange column chromatography.The purity and molecular weight of TBPs were determined by high performance gel filtration chromatography(HPGFC).Polysaccharide samples were hydrolyzed with sulfuric acid solution,and derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP).The monosaccharide composition of the hydrolysates obtained was analyzed by RP-HPLC.The results showed that TBPs could be obtained by water extraction and alcohol precipitation after protein removal by Savag method.Three polysaccharides,named as TBP-1,TBP-2 and TBP-3,were obtained by DEAE-cellulose column chromatography with molecular weights of 144544,445656 and 636795,respectively.Liquid chromatographic analysis based on pre-column derivatization showed that TBP-1 and TBP-2 were composed of glucose and TBP-3 of mannose,rhamnose,glucuronic acid,glucose,galactose and arabinose in a molar ratio of 4.32:2.41:1.00:39.8:9.64:2.02.