Effects of Chitosan-TPP Nanoparticles on Wound Healing of Hepatic Tissue after Severe Bleeding

Objective: To study the effects of chitosan-TPP nanoparticles on wound healing by building the model of severe bleeding of SD rat's liver. Methods: 24 SD rats were randomly divided into three different groups, each group included 8 rats. The rats in the negative group and the chitosan-TPP nanoparticles group(NP group) was dealt with severe bleedingin the liver. In the NP group, the chitosan nanoparticleswere sprayed onto the wound until the whole wound was sealed. In the negative group, the same procedure was performed except any treatments. 15 days after operation, the microstructure of hepatic tissues in both NP group and negative group were investigated by SEM and TEM. Results: It was found in the histological observation that new granulations and a large amount of collagens were generated when chitosan-TPP nanoparticles was treated on the severe wound, which is one of the main signs in the early period of wound healing. Conclusions: The chitosan-TPP nanoparticles can be an ideal material in the treatment of severe bleeding and subsequent wound healing.