The optimal conditions of extracting polysaccharide from Flammulina velutipes

The optimal extracting conditions of polysaccharide from fresh Flammulina velutipes is studied.The extracting times,time,temperature and pH are discussed.The experimental results show that the optimal extracting conditions of polysaccharide from fresh Flammulina velutipes are adding H2O volume 30 times as much as sample, 90 ℃ for 1.5 h with pH 8, the yield of extracting polysaccharide is 0.833%.Some conditions,the yield of polysaccharide is up to 1.250% when the ultrasonic is used for 20 min.