2010•Journal of Wuhan UniversityRequires access

Construction of a Fosmid Library of Litopenaeus vannamei and Screening of a Calcium-Release-Channel Gene

Xiaohan Chen

Abstract

A fosmid genomic library was constructed for Litopenaeus vannamei.The library consisted of about 1.1×105 clones,in which average length of inserts was greater than 35 kb.The library was screened by PCR analysis using primers designed according to the EST from L.vannamei homology to calcium-release-channel gene of Drosophila melanogaster.A positive clone was selected,subjected to DNA sequencing,and an existence of the calcium-release-channel gene was revealed.These results will improve the study on gene cloning and molecular breeding for L.vannamei.

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Key concepts: Fosmid, Genomic library, Gene, genomic DNA, Biology, Calcium channel, Cloning (programming), Molecular cloning