Preparation and Certification of Reference Materials of Turpentine Oil from Pinus kesiya var.langbiangensis

The reference material of turpentine oil from Simao pine(Pinus kesiya var.langbiangensis) oleoresin were used to investigate its preparation process,homogeneity,evaluation of stability and certification.Simao pine is the major tapping pine species in Yunnan Province.The turpentine oil was extracted from simao pine oleoresin using a steam distillation method,and the distillate was characterized by a gas chromatographic area normalization.The homogeneity test showed that reference materials of turpentine oil kept stable for 36 months or longer.The certification results showed that the standard content values of 3 indicating components were: α-pinene XS=75.30 % with standard uncertainty UA=0.23 %,extended uncertainty U0.95=0.45 %;β-pinene XS=17.80 %,UA=0.08 %,and U0.95=0.16 %;β-phellandrene XS= 3.50 %,UA=0.08 %,U0.95=0.16 %,respectively.