Determination of Carbamate Pesticides in Animal-Derived Foods with Accelerated Solvent Extraction and Gel Permeation Chromatography by High Performance Liquid Chromatography Post-Column Derivation

Objective To determine the multi-residues of 12 kinds of carbamate pesticides in animal-derived foods.Method The samples were extracted with acetonitrile by accelerated solvent extraction(ASE)and cleaned up by auto gel permeation chromatography(GPC)and primary secondary amine(PSA)packing material.The collected solution was analyzed by high performance liquid chromatography-fluorescence detector(HPLC-FLD)with post-column derivation and quantified by external standard method.The wavelengths of excitation and emission were set at 330 nm and 465 nm,respectively.Results The limits of detection(S/N=3)of 12 kinds of carbamate pesticides ranged from 0.24 μg/kg(isoprocarb)to 1.02 μg/kg(aldicarb sulfoxide).The recoveries ranged from 62.1%±8.8% to 104.0%±5.6% by fortified with 0.05 mg/kg,0.1 mg/kg and 0.2 mg/kg of 12 kinds of carbamate pesticides into the blank treated samples.The RSD was less than 20%.Conclusion The limits of detection and the recoveries of 12 kinds of carbamate pesticides both meet the analysis requirements of pesticide residues.