Effect and mechanism of Jiangu Guben capsules on postmenopausal osteoporosis of ovariectomied rats

OBJECTIVE To investigate the effect of Jiangu Guben (JGGB) capsule on postmenopausal osteoporosis in ovariectomized female rats.METHODS Sixty female Sprague-Dawley rats without pregnancy and delivery were used,50 of them were ovariectomized and randomly divided into 5 groups:ovariectomy (OVX),OVX with JGGB capsule 0.8 g·kg-1,0.4 g·kg-1,and 0.2 g·kg-1,and OVX with Xianlinggubao capsule 0.5 g·kg-1,the others 10 rats were sham-operated group.The rats were treated on the fifth day after the operation and continuated for 12 weeks.At the end-point of the experiments,the bone mineral density and bone mineral capacity were assayed by double energy X-ray bone-densitometry equipment,the blood was collected to detect the activity of StrACP in serum,and the hydroxyproline contents of femur by commercial kits,the bone's biomechanics of femur were assayed.RESULTS Capsules decreased remarkably the activity of StrACP in serum and increased significantly the contents hydroxyproline of femur,increased significantly the bone mineral density and bone mineral capacity,and improved the bone biomechanical property.CONCLUSION JGGB capsules can effectively prevent the postmenopausal osteoporosis of ovariectomied rats,the mechanism maybe involved in ameliorated biomechanics and increased the bone mineral capacity and the content of collagen in bone.