Determination of sulfaclozine sodium in feed by high performance liquid chromatography

A high performance liquid chromatography(HPLC)method,a standard determination of sulfaclozine sodium in feeds,was established in this study,after extracted by acetonitrile,concentrated,dissolved with 2 mL methanol+8 mL 0.05 mol·L-1 KH2PO4 and cleaned by C18 SPE column,filtrated with 0.2 μm organic phase filter,then detected with Agilent 1100 liquid chromatography.The determination conditions were as follows:with C18 column,1.000 mL·min-1 of flow rate,25 ℃ of column temperature,UV detection wavelength under 272 nm,mobile phase by acetonitrile and 0.2% phosphoric acid volume ratio 35∶65.The results showed that the chromatographic peak retention time of sulfaclozine sodium chloride was 7.5 min.By the adding conditions of 0.5,1,2 μg·mL-1 of sulfaclozine sodium,the recovery rate was between 90.6%-97.1%.The limit of detection was 0.5 mg·kg-1.The limit of quantification was 1.0 mg·kg-1,the linear range was 0.50-10.00 μg·mL-1,Y=57.332X+2.972 9(R2=0.999 3),and the relative standard deviation(RSD)of four kinds of feed samples were 1.0%-2.9%.The results indicated that the HPLC method has a high sensitivity,good accuracy and precision,and it can be used for detecting residual feed sulfaclozine sodium effectively.