Dose-response relationship of oxidized low density lipoprotein on human umbilical vein vascular endothelial cells damage in vitro

Objective:To explore the dose-response relationship of oxidized low density lipoprotein(ox-LDL) on human umbilical vein vascular endothelial cells(HUVEC) damage in vitro. Methods: HUVEC were treated with different concentrations of ox-LDL for 24 h.Morphological change was observed. And then cell viability was measured by MTT and growth inhibition ratios were calculated. The content of MDA in endothelium cells was measured. Results: With the increase of the concentration of ox-LDL, cells morphology of HUVEC was deteriorated. Cells became loose, broken and eventually dead. When cells were exposed to different doses of ox-LDL for 24 h, compared with control group, cell viabilities of 50, 100 and 200 μg/m L groups were decreased respectively and the differences were statistically significant(P0.05). The statistical result of MDA concentration in cells after 24 h was consistent with MTT. The growth inhibition ratios for50, 100, 200 μg/m L group were 23.2%, 45.2%, 62.0%. Conclusion: When HUVEC are exposed to ox-LDL for 24 h, the cell viability decreases with the rise of the ox-LDL concentration while the cell growth inhibition rate and MDA content in the cells increase. When the concentration of ox-LDL is below 200 μg/m L, the HUVEC suffer marked oxidation damage and present dose effect relationship.