Suppressive effects of miR-519din bladder cancer cell line 5637 by targeting OCT4

Objective:To investigate the mechanism of suppression of miR-519din bladder cancer cell line 5637.Method:After transfected with miR-519d mimics in bladder cancer cell line 5637,the proliferation of 5637 cells were examined by MTS assay and colony formation experiment.Western blot assay was used to analyze the expression of OCT4in transfected cells,and the relative luciferase activity was detected by dual luciferase reporter system.OCT4-specific siRNA was used as a positive control.Result:The proliferation and colony formation potential of 5637cells transfected with miR-519d mimics were significantly lower than control group.The relative luciferase activities of OCT4-3'UTR/miR-519dco-transfected group were significantly lower than other groups(P 0.05),and the expression of OCT4has been down-regulated by miR-519d transfection.And the effects of OCT4-specific siRNA on cell proliferation and colony formation complied with that of miR-519d.Conclusion:It is indicated that miR-519dsuppresses cell growth of bladder cancer cell 5637 directly through targeting OCT4.